Problem:
Question: What would be the best way to separate the follwoing mixture of peptides for use in bioassays and why? What is their expected order of sparation after each technique? Explain.
a) CTRNYLAADTTNR
b) QERKLPAIDEDDE
c) ACSKWQLIMHSTN
d) NTAKFIVLDTEER
I know that a has a net charge of +1, b has a net charge of -4, c has a net charge of +2, and d has a net charge of -1. I don't know the best set of techniques to use (i.e. ion-exchange chromatography, size-affinity, affinity-binding, etc.)
Any help would be greatly appreciated!