Problem:
Question: Pravastatin is a cholesterol lowering drug whose mechanism of action is the inhibition of HMG-CoA reductase. If a patient takes a 40.0 mg dose of pravastatin, what is the concentration of pravastatin in the blood? Assume the patient has 5.0 L of blood. The molecular weight of pravastatin is 425.
___ uM
Question: Pravastatin is a competitive inhibitor of HMG-CoA reductase with a Ki of 2.3 × 10-3 uM. What fraction of the enzyme will have a bound substrate under the conditions described above? Assume the concentration of HMG-CoA is 15 ?M, and the KM for HMG-CoA reductase is 1.4 uM.
fES = ___
Question: After a period of time, all but 0.10% of the pravastatin in the blood is metabolized. What fraction of the enzyme will have bound substrate under these conditions?
fES = ___
Please provide correct answer.