Explain Isolation of RNA
To isolate RNA you frequently use a protein denaturant solution of guanidinum salts. These chaotropic explanations denature by disrupting H- bonding. The solutions as well contains a reducing agent to denature disulfide bonds. The final composition of the solution is: 4 guanidine isthiocyanate (GITC), 25mM sodium citrate, pH 7.0 , 0.5%(w/v)SDS, 100mM β-mercaptoethanol. You have guanidine isothiocyanate powder (mol.Wt.118.16), 1 M NaCitrate, pH 7.0, powder SDS and b-mercaptoethanol (density 1.12 g/mL, mol. Wt.78.1). Exactly how would you make up 100 mL of this solution?