(a) Molecule X receives excitation as well as emits fluorescence. If the excitation wavelength is 320 nm, which of the subsequent could be the emission wavelength 270 nm, 290 nm, 320 nm, 350 nm? Explain your result.
(b) Explain how the following factors affect fluorescence quantum efficiency- increasing structural rigidity, decreasing temperature, rising sample concentration.
(c) Explain which method is more sensitive fluorescence or absorption spectroscopy? Define your result.
(d) Define the difference between fluorescence and phosphorescence.