Problem: Hello! So you can choose to watch the video that i will place below for more information, then if you can please put down a detailed description of the steps involved in isolating and inserting a foreign gene into a bacterial expression system. Can you also kindly explain to me how to express the protein after the gene is inserted?
Can you kindly make sure to include the following topics:
1. Explain how a foreign gene is obtained
2. Describe how to insert a gene into a bacterial genome or plasmid
3. Describe how to express the protein coded by the gene
4. Discuss the limitations of using bacterial expression systems for large/complex proteins
5. What other expression systems can be used to circumvent the issues found with bacterial expression systems?
YouTube Video: Bacterial Transformation