Explain about the Microcolony DEFT?
It allows determination of viable cells. Food homogenate is filtered through a DEFT membrane and then placed on the surface of appropriate culture medium plate. Microcolonies develops and are counted with a microscope. Another variation is a microcolony epifluorescence microscopy where acridine orange is used for staining and the microcolonies are enumerated by epifluorescence microscopy. Results are obtained within 6-12 hours.