Experiment in lab to reconstitute purified bacteriorhodopsin


Experiment in the lab, you are asked to reconstitute purified bacteriorhodopsin, a light-driven H+ pump from the plasma membrane of photosynthetic bacteria, and purified ATP synthase from ox-heart mitochondria together into the same membrane vesicles (Figure). You are then asked to add ADP and Pi to the external medium and shine light into the suspension of vesicles.

You tell a friend over dinner about your new experiments, and he questions the validity of an approach that utilizes components from so widely divergent, unrelated organisms: "Why would anybody want to mix vanilla pudding with brake fluid?" Defend your approach. Include the basic principle of the mechanism in this experiment 

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Biology: Experiment in lab to reconstitute purified bacteriorhodopsin
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