Estimate the Vmax and Km of the enzyme catalyzed reaction from both of the plots. Compare your results from the two methods and explain why they are slightly different.
[S] (µmol/L) Vo (mM/min)
0.05 0.22
0.1 0.34
0.2 0.53
0.4 0.74
0.8 0.91
1.6 1.04
3.2 1.16
[S] vs Vo
Vmax = 2.138
Km=-4.31
Lineweaver-Burk plot
Vmax=1.1326
Km= -5.316