Question: A microcalorimetry experiment was done on spinach plants, following the same protocol that we discussed in lecture. Several spinach plants were "control" plants, i.e. nothing unusual was done to them. However, several plants were treated with a particular pesticide for several days. Leaves from the control plants were placed into ampule #1. Leaves from the pesticide treated plants were placed into ampule #2. The microcalorimetry experiment was performed just as we discussed in class: in part "A" 40 pl of water was placed in the well; in part "B" 40 [.11 of 0.4 M NaOH was placed in the well; in part "C" 40 u] of water was placed in the well.
Now let's plot the results of this experiment. Make a graph; label each axis. Draw in carefully the results ?om ampule #1: a heat rate of 75 uW/mg in part "A"; a 20% increase in heat rate when 0.4 M NaOH is added to the interior well in the ampule. What should part "C" look like for ampule #1?
What would the plot look like for ampule #2 if the same mass of spinach leaves treated with pesticide gave a 30% decrease in heat rate/mg in part "A" (relative to part A for ampule #1); and the same heat rate/mg in part "B" as was seen in part "A" for ampule #2? What should part "C" look like for ampule #2?
What do you conclude about the effects of the pesticide on the metabolism of the spinach leaves?