Problem:
I just wanted to understand the basic steps behind the replication of the lagging strand of DNA:
Have helicase unwind it first
- DNA Primase lays down RNA primers in fragments, called Okazaki fragments
- DNA polymerase III goes through and corrects all the mistakes (essentially replace the Uracil with Thymine)
- DNA polymerase I goes through and removes RNA primer
- DNA ligase hooks up each of the 5' to 3' end fragments
Required:
Question: Is this the correct order of steps?
Question: Does the leading strand need any RNA primer or does the DNA polymerase just start and go to the end without any help?