Discussion:
Enzyme Kinetics & Steady State
For a MM reaction
k1=5x10^7
k-1=2X10^4
k2=4x10^2
i calculated Km and Ks to be equal at about 4 x10^-4M
Does substrate binding achieve equilibrium or the steady state?
Could you clarify when you have ph=pkA how you get whether it is -1/2 or +1/2 or in the other case +1/3 or -1/3. How do you tell whether it is + or -