Dna sequencing and technology
1) Explain DNA sequencing with reference to the cloning of a DNA fragment in bacteriophages M13 based cloning vector.
2) Explain the use of an expression vector to isolate the gene from a cDNA library that codes for a particular protein.
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Whenever constructing cDNA libraries it is much significant to copy the whole of an mRNA into cDNA. One way to try and make sure that the 5' end of an mRNA is represented in a cDNA copy is to use "cap-trapper" technology.
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Explain DNA sequencing with reference to the cloning of a DNA fragment in bacteriophages M13 based cloning vector.
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