Assignment:
Ten grams of hamburger were added to 90 mL of sterile buffer. This was mixed well in a blender. One-tenth of a mL of this slurry was added to 9.9 mL of sterile buffer. After thorough mixing, this suspension was further diluted by successive 1/100 and 1/10 dilutions. One-tenth of a mL of this final dilution was plated onto Plate Count Agar. After incubation, 52 colonies were present. How many colony-forming units were present in the TOTAL 10 gram sample of hamburger?
Provide complete and step by step solution for the question and show calculations and use formulas.