A. List three reasons why you might choose to stain a particular slide rather than view it as a wet-mount.
B. Define the following terms: Chromophore: Acidic Dye: Basic Dye:
C. What is the difference between direct and indirect staining?
D. What is heat fixing?
E. Why is it necessary to ensure that your specimens are completely air dried prior to heat fixing?
F. Describe what you observed in your plaque smear wet-mount, direct stained slide, and indirectly stained slide. What were the similarities? What were the differences?
G. Describe what you observed in your cheek smear wet-mount, direct stained slide, and indirectly stained slide. What were the similarities? What were the differences?
H. Describe what you observed in your yeast wet-mount, direct stained slide, and indirectly stained slide. What were the similarities? What were the differences?
I. Were the cell types the same in all three specimen sets: yeast, plaque, and cheek? How were they similar? How were they different?