Discussion:
Determining the sequence of a peptide after digestions
A.In trying to identify the amino acid sequence of the plant hormone of a 12-mer peptide;
The following were found:
1.One of the chymotrypsin digestion products was identified as RGDTY
2.Trypsin digestion of the 11-mer peptide gives GQLFR and another 6-mer peptide
3.Cyanogen Bromide (CNBr) gives an 11-mer peptide and homserine lactone
4.Carboxyl peptidase C first released Glu and then released Ile, then Tyr
Identify the sequence of this peptide?
B.When cysteine is treated with bromoethylamine (BrCH2CH2NH3+), the resulting modifies side chain can be recognized by trypsin.
DRAW the structure of the modified side chain of cysteine by bromoethylamine and EXPLAIN WHY trypsin can recognize it?