As a result of the reaction, 0.03 mole/liter of H+ was produced. An enzyme-catalyzed reaction was carried out in a 0.1 M TRIS (pK =8.1) buffer at pH 7.8. TRIS+ and TRIS0 represent the acidic and basic forms of TRIS, respectively.
1. Before the reaction was initiated, what was the ratio of TRIS+ and TRIS0?
2. At the end of the reaction what was the ratio of TRIS+ and TRIS0?
3. Determine what was the pH at the end of the reaction?