Problem: As a research investigator you are interested in studying the functional properties of the P2X7 receptor. So the first step you plan is to overexpress P2X7 receptor in HEK293 cells. Following this step, you are now requested to answer the following questions:
Design an assay to Quantitate the number of cells that are overexpressing the P2X7 receptor. Based on the assay that you choose to design, describe the assay design, endpoint read, types of controls you will need to include and the expected outcome/results of your assay to determine what % of your cells are overexpressing P2X7
- Assay Design, instrumentation and readout
- Controls
- Expected Result/Quantitation