Q1) Determine the weight in grams of acetic acid (FW = 60.05) and Na Acetate (FW = 82.03) needed to prepare 1 L of a standard buffer at pH 4.20 with a total acetate concentration of .25 M. (pka=4.76)
Q2) Three polypeptides listed below are in a mixture. Of the three, which would migrate most rapidly (i.e. elute first) through an anion-exchange resin at pH 7.0?
ATKNRASCLVPKHGALMFWRHKQLVSDPILQKRQHILVCRNAAG
GPYFGDEPLDVHDEPEEG
PHLLSAWKGMEGVGKSQSFAALIVILA