Question - Three polypeptides listed below are in a mixture. Determine the total charge for each peptide at pH 7.0, then, of the three, which would migrate most rapidly (i.e. elute first) through a strong anion-exchange resin at pH 7.0?
ATKNRASCLVPKHGALMFWRHKQLVSDPILQKRQHILVCRNAAG
GPYFGDEPLDVHDEPEEG
PHLLSAWKGMEGVGKSQSFAALIVILA