A 10x concentrated stock of the running buffer contains 144 g glycine, 30.3 g Tris base and the 10g SDS. These components are dissolved and brought up to a total volume of 1L.
1. Determine the volume of 10x stock is added to what volume of water to make one liter of a 1x working solution of the running buffer?
2. Determine the final concentration of all three components in the 1x working solution? Report Tris base and glycine in m Mand SDS as w/v percent.