Problem
I. Determine the expected ratio of wild-type, heterozygous and homozygous pups from the breeding scheme.
II. Show where the restriction enzyme SfcI will cut in the GABRD and GABRD I284T PCR product sequences
III. Explaining the difference between the two antibodies, anti-p44/42 MAPK and anti-phospho-p44/42 MAPK, what they will be measuring, and why.