Discussion:
Determine Km and Vmax and determine what types of inhibition
1. Data from enzyme kinetic studies of an enzyme at specific substrate concentration. Data below also include from studies with town different inhibitors
[S] (mM) Vo (uM/sec) Vo (uM/sec) Vo (uM/sec)
w/o inhibitor w/inhibit A w/inhibit B
1.3 2.5 1.17 0.62
2.6 4.00 2.10 1.42
6.5 6.3 4.0 2.65
13.0 7.6 5.70 3.12
26.0 9.0 7.20 3.58
2. Determine the Km and Vmax for the enzyme not inhibited. Plot all. What types of inhibition is displayed by inhibitor A and B? Would these inhibitors have an affinity for ES or E?
3. Everytime I plot this it is a curved line upside down. I do not know what I am doing wrong??? Please give me step by step. I am a very visual person.