determination of vitamin b12spectrophotometric, Biology

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determination of vitamin b12spectrophotometric

Determination of vitamin B₁₂

Spectrophotometric determination of the characteristic ultraviolet absorption is suitable for the determination of pure vitamin B₁₂ or of pure aqueous solution in the absence of interfering impurities. Microbiological assay is performed by any of the following:

Determination of the diffusion in the cup-plate test using a mutant of Escherichia coli ATCC 10799 as the test organism
Titrimetric determination of the acid produced in the tube test using Lactobacillus lecihmannii ATCC 7830.

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Biology: determination of vitamin b12spectrophotometric

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