When you calibrated the same gel filtration column, the elution volumes of proteins of known molecular weights were as follows: 400,000 Da, 10 ml; 250,000Da, 20 ml; 150, 000 Da, 30 ml; 100,000 Da, 40 ml. Suppose you perform gel filtration and measure an elution volume of 25 ml. Design the logarithm of the molecular weights of the known protein versus the elution volume.