Problem: Botox has become popular for reducing wrinkles. It is a dilute solution of a toxin which inhibits skeletal muscle contraction by working at the presynaptic portion of the neuromuscular junction, where acetylcholine (ACh) is the neurotransmitter.
Which of the following options could be a potential mechanism for how Botox works? Explain your reasoning.
- Inhibits pre-synaptic voltage-gated calcium channels
- Blocks post synaptic receptors preventing ACh binding
- Inhibits vesicular release of ACh
- Decreases ACh esterase activity