Problem:
Question: Which of the following is a way in which the mechanism by which topoisomerase I breaks a DNA strand differs from the mechanism of phosphodiester bond formation? Can be multiple answer.
a) Only one involves an OH group acting as a nucleophile
b) Only one involves the use of ATP
c) Only one involves a nucleotide-amino acid bond
d) Only one involves the release of a pyrophosphate molecule
e) Only one requires DNA Polymerase
Give reasoning with your answer.