Problem:
The membrane potential across the inner mitochondrial membrane is -60mV (negative inside), and the external side of this membrane has a ph that is 1.4 units below the pH on the internal side of this membrane(i.e.More acidic externally.
Required:
Question 1: how much free energy is produced if one mole of H+ is transported into the mitochondrail matrix under these conditions?
Question 2: Under steady-state condtions in a cell, the free energy change for hydrolysis of ATP is approximately -11.5 kcal/mol.How many moles of H+ must be transported across the membrane to provide energy for the sythesis of one mole of ATP?
Show the pedigree and your calculation.