1. Which sera samples reacted with the antibody and which did not. Explain why a difference was observed.
2. Estimate the lowest concentration of IgG that you detected in your assay. (Hint: The serum concentration of IgG is about 10 mg per ml and the concentration of serum in Well #1 is 1%.)
3. Describe an ELISA procedure that you would use to determine if a person was infected with the human immunodeficiency virus, the causative factor of AIDS.