Describe a situation where you might need to cut your vector
Problem
Describe a situation where you might need to cut your vector and the insert you intend to ligate into your plasmid with different restriction enzymes? What are these enzymes called?
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Describe a situation where you might need to cut your vector and the insert you intend to ligate into your plasmid with different restriction enzymes?
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Identify the three processes and give a couple of examples what could go wrong in each of them.
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