Derive the Michalis - Menton equation using the steady-state approximation. Show all your steps.
Use a program like Excel to draw a plot (hint: x axis: 1/S, y axis: 1/v ) and calculate the Km and vmax for the following data.
Draw a plot and calculate the Km and vmax using the data in column vA. Then calculate Km and vmax using the data in column vB. (hint: A is for substrates only, B is for substrate with inhibitor).
For above question, what type of inhibitor was used? Explain by drawing the potential structure of enzyme and where the inhibitor is binding.