Define Regents for Estimation of Vitamin C - Colorimetric Method?
1. 2, 4 dinitrophenylhydrazine reagent - Dissolve 2 g of dinitrophenyl hydrazine in 100 ml of 9N sulphuric acid and allow to stand overnight. Mix and use.
2. 5% thiourea solution - Dissolve 25 g of thiourea in 500 ml of distilled water.
3. 9N sulphuric acid
4. 85% sulphuric acid
5. 0.6% copper sulphate
6. Colour reagent or Dinitrophenylhydrazine - thiourea - copper sulphate (DTC) reagent - Mix
7. 5% trichloroacetic acid (TCA)
8. 5% metaphosphoric acid in 10% acetic acid solution - Mix 1000 ml of 5% metaphosphoric acid and 500 ml of 10% acetic acid.
9. Ice for cooling.