Construct a full-spectrum least-squares fit to the tissue

Question

Hemoglobin spectroscopy.

There are three files to be used for this problem: oxy.asc gives the μa spectrum of 100% oxyhemoglobin for tissue with 0.5% blood content by volume and 150 mg of hemoglobin per mL of blood (first column is wavelength in nm, second column is μa in cm-1), deoxy.asc gives the same for 100% deoxyhemoglobin, and tissue.asc gives a spectrum of tissue with unspecified blood content and oxygenation. Each spectrum is given between 700 and 950 nm.

Using Matlab, Construct a full-spectrum least-squares fit to the tissue spectrum. Using these fitting coeffcients for oxy and deoxy hemoglobin, calculate

(a) the O2 saturation percentage and

(b) the micromolarity of hemoglobin in the tissue as a whole (not just the blood). Plotyour least-squares spectral fit on top of the sample spectrum, to demonstrate that it is a good fit.

Attachment:- Assignment.rar

Answer 1

This assignment is to be done by using Matlab, related to full-spectrum least-squares fit to the tissue spectrum. It encompass three files to be employed for this problem: oxy.asc provides the µa spectrum of 100% oxyhemoglobin for tissue having 0.5% blood content by volume and 150 mg of hemoglobin per mL of blood (i.e. the Ist column is wavelength in nm, IInd column is µa in cm-1), deoxy.asc provides similar for 100% deoxyhemoglobin, and tissue.asc provides a spectrum of tissue having unspecified blood content and oxygenation. Each spectrum is provided between 700 and 950 nm. Construct a full-spectrum least-squares fit to the tissue spectrum by Matlab. By using such fitting coeffcients for oxy and deoxy hemoglobin, compute: a) The O2 saturation percentage b) The micromolarity of hemoglobin in the tissue as a whole (not just the blood). Plot at least-squares spectral fit on top of the sample spectrum, to explain that it is a good fit.