Assignment:
The enzyme succinoxidase adds oxygen to succinate to give fumarate. The reaction follows Michaelis-Menton kinetics. The following rate data were determined for a solution in which the enzyme concentration is 10.0 uM:
[S](mM) ----------------- 0.33 10.00
vo (uM s-1) at 20 C ----- 0.50 1.17
vo (uM s-1) at 40 C ----- 2.41 5.65
(a) Determine Vmax and Km for the reaction at 20 C and 40 C.
(b) Compute the activation energy of this reaction (succinate + O2 --> fumarate).
(c) Will the activation energy be higher or lower in the absence of succinoxidase? Why?
Provide complete and step by step solution for the question and show calculations and use formulas.