Computing productivity of chemostat-monod kinetics applies

Question

1) Write in detail about different techniques used in cell number density determination   

2) Compute the productivity (i.e., DP) of a chemostat under the following conditions:

i) Suppose Monod kinetics applies.

ii) Suppose the Luedeking – piret equation for product formation (q_p=αµ_g+β) applies

iii) Suppose steady state:

D = 0.8µm                       Y^M_X/S =0.5g X/g S            µ_m =1.0 h-1
S₀ =1000 mg/L               α = 0.4 mg P/g X             β =0.5 h-1 mg P/g X
K_s =10 mg/L.                                               

3) For an ideal chemostat, prove that: 1/Y^AP_X/S = 1/Y^M_X/S + m_s/D.           

4) Describe the unstructured model for microbial growth           

5) Discuss about the bioreactor considerations for suspension culture of plant cells.