1. Complete the following purification table. Which step gives the best purification?
2. Define and/or explain the significance of All of the following.
a. Explain why a fluorescent amino acid, whether naturally occurring (tryptophane) or artificial (Aladan), might be of advantage in Biochemistry.
|
Step
|
Total VoIn me
|
Total Protein (mg)
|
Total Activity (units)
|
Specific
Activity
(units/mg)
|
Yield (%)
|
Purification
(n-fold)
|
|
Crude Cell extract
|
2,800
|
70,000
|
2,700
|
|
|
|
|
Ammonium Sulfate
|
3,000
|
25,400
|
2,300
|
|
|
|
|
Heat Treatment
|
3,000
|
16,500
|
1,930
|
|
|
|
|
DEAE Chromatography
|
80
|
390
|
1,630
|
|
|
|
|
CM Cellulose Chromatography
|
50
|
47
|
1,350
|
|
|
|
|
Bio-Gel A Chromatography
|
7
|
35
|
1,120
|
|
|
|