Cloning of dna segments in plasmid vector

Question

Question 1:

Write down the principle features of the pUC cloning system.

Question 2:

Illustrate out the term DNA modifying enzymes. Provide example.

Question 3:

Critically illustrate out the term expression vectors. Provide example for mammalian expression vectors.

Question 4:

Provide the significance of fusion proteins. Write down the vectors employed for the gene fusion.

Question 5:

Write down the steps in the cloning of the DNA segments in plasmid vector by poly dA: poly dT tailing technique?

Question 6:

Make a distinction between pGEM 3Z and pBR327.

Question 7:

Delineate invitro packaging.

Question 8:

Illustrate out the term transfection.

Question 9:

How could you raise the activity of plant promoter.

Question 10:

Write down the modifications which required converting a Ti plasmid in to a cloning vector.