Cloning of a dna fragment
1) Explain DNA sequencing with reference to the cloning of a DNA fragment in bacteriophages M13 based cloning vector.
2) Explain the use of an expression vector to isolate the gene from the cDNA library which codes for a precise protein.
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One of these dogs ate some of a birthday cake. Digestion and electrophoresis of DNA found at the scene of the crime with the restriction enzyme Scs1 revealed a banding pattern included of four bands. Which dog was most probable not responsible for
Why does project planning generally come before analysis and design? Write down some of the methodologies generally used for requirements elicitation?
Please discuss how relationships are created and enhanced in e-business environment. Critically discuss the potential risks of using Web 2.0 tools. Provide several examples.
Critically discuss the issues, challenges, or disadvantages to forming a strategic alliance (focus on supply chain).
Explain DNA sequencing with reference to the cloning of a DNA fragment in bacteriophages M13 based cloning vector.
You add up 10 ng of pGEM to 50 ul of competent cells, and then dilute this into a total of 1 ml Luria broth after the transformation. You take 100 ul of the Luria broth and plate on and agar plate. The next day, there are 780 colonies on the plate
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Restriction endonucleases are enzymes that digest the DNA. They are not similar digestive enzymes that completely digest DNA to monomers so cells can eat nucleic acids.
How many natural diamonds are for sale at moment? Note the wide array of sizes and prices of the diamonds.
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Evidence of Cultural Impact: The cultural impact of economic factors can be observed through specific practices and initiatives within the NSHA:
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