A CHO mammalian cell culture is set up by a chemostat stirred tank bioreactor to produce HBsAg. Glucose is the growth rate limited substrate and cell growth follows the Monod Model.
The feeding flow rate is 20L/hr with the glucose concentration 0.25M, and the glucose concentration in the outlet is 5g/L. It has been found out the maximal cell growth rate is 0.2 hr-1 and the saturation constant, or half-velocity constant of substrate is 2.0 g/L. The cell yield coefficient is Y X/S = 0.30g/g. Find out:
- CHO cell concentration in the outlet stream.
- Culture medium volume in the bioreactor at flow rate of 20L/hr.
- Keep the culture medium volume from b) constant, what is maximal flow rate to avoid the cells washed out from this bioreactor?