Case History: Mapping a Bacterial Chromosome
Background
Conjunction mapping can be used to locate genes on a bacterial chromosome. In this example, the F+ strain is able to synthesize methionine, valine, leucine, and histidine. The F- strain is unable to synthesize these amino acids; therefore, they must be supplied in the growth medium. Use the results from replica-plating to answer the questions.
Questions
1. On which media, if any, should the F+ be able to grow? The F-?
2. Identify the prototrophs and auxotrophs. How can you tell?
3. Can you map the chromosome of this species? What further information do you need?