Calculate:-
(a) the velocity and
(b) the degree of inhibition of an enzyme catalyzed reaction in the presence of 6x10-4 M substrate (KM = 10-3 M) and 2.5x10-4 M of a non-competitive inhibitor (Ki = 3x10-5). Vmax = 515 nmoles/L/min.
vi = __._ nmoles/L/min
% Inhibition = __._%