Calculate the concentration given the absorbance. Each test tube has 5ml of water then a certain amount of bromol blue. 1 ul bromol blue absorbs at= 0.092, 2 ul=0.105, 4ul=0.093, 10=0.199, 20= .225, 30= 0.592, 50=0.957, 100= 1.717. All absorbances measured at 540 nm. I have been told that I need to use C1V1=C2V2 but I have no idea how.