Discussion:
To calculate protein concentration with given absorbance
Question1.
The data below was obtained in an experiment to determine the protein concentration of two unknown solutions. Analyse the data, plot a suitable graph and determine the protein concentrations of the unknowns
Protein standard (mg cm-3) Absorbance at 540nm
0 0.00
1 0.122
2 0.243
3 0.365
4 0.468
5 0.588
Unknowns Absorbance in triplicate
1 0310, 0.318, 0.322
2 0.444, 0.423, 0.443
Question 2.
State two things which may result in error in this type of experiment?
Write your answer/response in detail with examples and facts and figures using APA style of formatting, size 12, font Times new roman and answer must be single spaced