Problem
For an electrophysiology experiment, you will construct a concentration-response curve with currents elicited by increasing concentrations of the P2X2 receptor agonist, ATP. You have a 50.0 mg/mL adenosine 5'-triphosphate (ATP) disodium salt stock solution. From that stock, you must prepare through serial dilution on a 10-fold scale 2 ml each of 1 uM to 1 mM ATP solutions in recording medium (previously prepared). Briefly describe the protocol for preparing the solutions, calculating the appropriate amounts of components to be mixed. (ATP MW = 507.18 g x mol-1; ATP disodium salt MW = 551.1 g x mol-1)