You now generate a NEW buffer solution by adding 0.1 mL of 10 M HCl to the solution in the previous question, how many mols of Tris-HCl would you now have in the NEW buffer solution?
Based on the Henderson-Hassalbach equation how many mmols of Tris-HCl are there in a 100 mL of a 100 mM Tris-HCl buffer solution at pH 8.1? Note that the 100 mM refers to the sum of Tris and Tris-HCl concentrations.