1. What is PCR, requirement to make a PCR reaction (buffer, taq polymerase, nucleotides...), the thermocycler
2. The different steps of a PCR reaction
3. What is STR, how to analyze STR profile
4. How many bands are formed when a plasmid is digested with one or several restriction enzymes.
5. How to analyze DNA digestion pattern Know DNA migration in agarose gel
6. DNA analysis by RLFP and STR