1. Transcribe the following DNA sequence:
Hb A:
5' AGT AAC GGC AGA CTT CTC CTC AGG AGT CAG GTG CAC CAT 3'
2. Translate your new RNA sequence using the genetic code. Remember that when determining your amino acid sequence, the RNA sequence is read from 5' to 3'.
Note: the first amino acid will be Met.
3. Transcribe the following DNA sequence.
Hb S:
5' AGT AAC GGC AGA CTT CTC CAC AGG AGT CAG GTG CAC CAT 3'
4. Translate your Hb S transcript.
Note: the first amino acid will be Met.
Post-Lab Questions
1. How long are each of your peptide chains (how many amino acids are present)?
2. What differences exist between your two transcripts? What about your translated product?
3. Hb A represents a section of the 626 base normal hemoglobin beta mRNA sequence. Hb S is from the sickle cell hemoglobin. Is the mutation found in sickle cell hemoglobin a point mutation or a frameshift mutation?
4. Does the sickle cell mutation result in a missense mutation, silent mutation or nonsense mutation? Why?