1) Is cytoskeleton involved in vacuole formation? If so what role does it play and what proteins are involved?
2) What are the advantage of using Tethrahymena as experimental system? What are the normal source of food for Tetrahymena? Explain the importance of vacuole formation in Tetrahymena life? Why did you use ink instead of water in the experiment?
3) What is the advantage for the eukaryotic cell to have a cytoskeleton instead of a rigid framework as the one present in bacteria?
4) How is the vacuole formation related to the presence of a dynamic cytoskeleton and dynamic membranes in eukayotic cells?