Assignment Help - homework help

  Transfer of genes to fertilized eggs

Transfer of genes to fertilized eggs or embryos:

The transfection of fertilized egg includes either the transfer of entire nuclei or entire chromosomes; or their fragments or the DNA segments.

A. For the transfer of entire nuclei, the cells are treated with cytochalasin B and enucleated. The enucleated cells are hatched with the desired karyoplasts (i.e., nuclei) for induction in the presence of polyethylene glycol (i.e., PEG).

B. For transfer of entire chromosomes, metaphase cells are subjected to hypotonic lysis and individual fragments or chromosomes are isolated and then incubated with entire cells or eggs for transfection.

C. Microinjection of DNA segments: In this the fertilized eggs are injected with DNA segments for the integration. DNA integrated eggs are then employed for acquiring transgenic animals.

D. Transfer of genes to cultured cells: In this stem cells are utilized. The stem cells are undifferentiated precursor cells. In such cultured cells, the gene can be delivered via vectors such as retroviruses or directly by methods like microinjection employing particle gun, electroporation or by the utilization of liposomes.

Transgenic animals have been generated in a variety of animals like rabbits, mice, sheep, goats, pigs, cows, fishes and so on.

 

Latest technology based Biology Online Tutoring Assistance

Tutors, at the www.tutorsglobe.com, take pledge to provide full satisfaction and assurance in Transfer of genes to fertilized eggs homework help via online tutoring. Students are getting 100% satisfaction by online tutors across the globe. Here you can get homework help for Transfer of genes to fertilized eggs, project ideas and tutorials. We provide email based Transfer of genes to fertilized eggs homework help. You can join us to ask queries 24x7 with live, experienced and qualified online tutors specialized in Transfer of genes to fertilized eggs. Through Online Tutoring, you would be able to complete your homework or assignments at your home. Tutors at the TutorsGlobe are committed to provide the best quality online tutoring assistance for Biology homework help and assignment help services. They use their experience, as they have solved thousands of the Biology assignments, which may help you to solve your complex issues of Transfer of genes to fertilized eggs. TutorsGlobe assure for the best quality compliance to your homework. Compromise with quality is not in our dictionary. If we feel that we are not able to provide the homework help as per the deadline or given instruction by the student, we refund the money of the student without any delay.
Home | Company Overview | Services | Discover Q&A | Sitemap | Contact Us | T & C | Refund Policy | Copyright Policy | Blog | Q&A | Directory

©TutorsGlobe All rights reserved 2022-2023.