In vitro fertilization (Test tube babies):
The so called test tube babies are formed by the method of in vitro fertilization. (In vitro = exterior the body, as against in vivo = within the body). This method is being increasingly utilized in couples who are not able to attain fertilization in the normal method.
Gonadotropins are managed to the woman to stimulate growth of follicles in the ovary. Just before ovulation, the ovum is eliminated (employing an aspirator) and is located in an appropriate medium. Spermatozoa are added to the medium. Fertilization and before time development of the embryo occurs in this medium.The procedure is carefully monitored. Whenever the embryo is at the 8-celled stage, it is place inside the uterine endometrium. Successful implantation occurs in around 20 percent of such trials. The method is complex and requires a team of well trained experienced personnel with very high degree of skill. The success rate is only around 20% and 2 or 3 attempts might be essential. It is also very costly. The first success with this method was attained by Steptoe and Edwards of UK in 1978. Though successful ‘test tube babies’ have been created in many countries.
Gamete Intra fallopian transfer (GIFT):
It is a newer technique in which the fertilized ova are initiated into the fallopian tube from where they naturally pass to the uterus for implantation. This technique is considered to have a higher success rate than IVF. An even newer method is the introduction of a single sperm into the ovum by microinjection.
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