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  Uses of Indirect Method

Uses of Indirect Method:

  • Indirect method can be employed for the detection of unknown antiserum with identified antigen.
  • It is also employed for the detection of unidentified organism employing known antiserum.

In bacteriology:

It is utilized for typing, identification, differentiation, quick diagnosis of bacteria and bacterial infections.

For Mycoplasma:

For explanation in tissue culture, Intra cellular position and for pleuro pneumonia similar to organism etiology of primary atypical pneumonia by FA method.

Chlamydia:

This test is employed for explanation and quantitation in infected cells.

In virology:

Immunofluorescent test is employed for the shown:

1. Recognition of viral antigens in different substrates.

2. Establishing the site in the host cell wherever the antigens are synthesized.

3. Recognition & quantitation of antibody in sera.

4. Dynamics of duplication of viruses.

5. Establishing the pathogenesis of few viral infections.

6. Etiological diagnosis of several human and animal viral infections.

 

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